- Training, Safety and Standards; and
- Mission Support.
The Operations directorate deploys aircraft, vessels, and personnel to air and marine branches and units throughout the country to provide rapid air and marine response capabilities.
- Northern Region is responsible for a nearly 4,000-mile boundary between the U.S. and Canada running from Washington State to Maine, including the Great Lakes region. It is comprised of the Bellingham Air and Marine Branch, Great Lakes Air and Marine Branch, and Manassas Air Branch.
- Southeast Region is responsible for over 2,000 miles of mostly water border on the southeast coast of the continental United States and Puerto Rico. It is comprised of the Houston Air and Marine Branch, Caribbean Air and Marine Branch, Jacksonville Air and Marine Branch, Miami Air and Marine Branch, and New Orleans Air and Marine Branch.
- Southwest Region is responsible for over 2,000 miles of border with Mexico, as well as along the western coast of the U.S. It is comprised of the El Paso Air Branch, Laredo Air Branch, McAllen Air and Marine Branch, San Diego Air and Marine Branch, Tucson Air Branch, Uvalde Air Branch, and Yuma Air Branch.
There are also two unique entities under Operations: National Air Security Operations (NASO) and the Air and Marine Operations Center (AMOC). NASO and AMOC are equivalent to a region.
AMOC is a state-of-the-art, multi-domain law enforcement and operations center located at March Air Reserve Base in Riverside, California. AMOC uses sophisticated technology to detect, identify, track, and direct the interdiction of suspect aviation and maritime targets in the Western Hemisphere. Under the Trade Facilitation and Trade Enforcement Act of 2015 (P.L. 114-25), the AMOC shall "manage the air and maritime domain awareness of the Department." AMOC integrates data from hundreds of domestic and internal radars and optical sensors. Satellite operations centers are located in Puerto Rico and Washington, D.C.
NASO is responsible for countering threats at and beyond the border using strategic aerial platforms such as the P-3 Orion maritime patrol aircraft and unmanned aircraft system. National Air Security Operations Centers are located in Jacksonville, Florida; Corpus Christi and San Angelo, Texas; Sierra Vista, Arizona; and Grand Forks, North Dakota.
The Training, Safety and Standards directorate is responsible for the oversight and implementation of AMO national training policies and procedures as well as overseeing the delivery of advanced aviation, maritime safety, law enforcement and leadership training. Within the directorate, an air training center is located in Oklahoma City, Oklahoma, and a marine training center is located in St. Augustine, Florida.
The Mission Support directorate is responsible for managing acquisitions and resources including people, money, and assets. Within the directorate, a marine center providing comprehensive vessel maintenance, logistics, and sustainment services for all CBP vessels is located in St. Augustine, Florida.