NEXUS lanes are located at the following land border crossings:
- Alexandria Bay, New York
- Blaine, Washington (Pacific Highway)
- Blaine, Washington (Peace Arch)
- Buffalo, New York (Peace Bridge)
- Calais, Maine
- Champlain, New York
- Detroit, Michigan (Ambassador Bridge)
- Detroit, Michigan (Detroit-Windsor Tunnel)
- Highgate Springs, Vermont
- Houlton, Maine
- Niagara Falls, New York (Lewiston Bridge)
- Niagara Falls, New York (Whirlpool Bridge)
- Pembina, North Dakota
- Point Roberts, Washington
- Port Huron, Michigan (Blue Water Bridge)
- Sault Ste. Marie, Michigan (International Bridge)
- Sumas, Washington